• TK Cheung Library (234 Anim Sci Bldg)
  •  Positive and negative controls in molecular labs  
    • Concept, importance, types, examples
  • Basics of DNA extraction 
    • Principle
    • Different types (manual, column-based, magnetic-bead based)
  • PCR optimization and troubleshooting  
    • Stages in a cycle
      • Reagents and their roles (comparison of different enzymes, hot vs. cold start); modifications
      • Inhibitors
      • Contamination
  • Primer design  
    • Length, Tm, Ta, GC content, secondary structures 
  • Gel electrophoresis  
    • Principle, different gels, different dyes, troubleshooting
  • RNA extraction and purification 
    • Differences with DNA extraction
  • Real time PCR  
    • Concept, optimization, 
  • qPCR, RT PRC and real time PCR
    • Measurement of nucleic acid quantity 
  • Comparing and contrasting Nanodrop, Qubit, PicoGreen and Bioanalyzer
    • Parameters to check, RNA vs DNA